研究成果

Kikuta, H.; Aramaki, T,; Mabu, S.; Akada, R.;Hoshida, H., The presence of an intron relieves gene repression caused by promoter-proximal four-bp specific sequences in yeast, BBA-Gene Regulatory Mechanisms, 1866(4): 194982, doi.org/10.1016/j.bbagrm.2023.194982, 2023

Nakamura, M.; Kikuta, H.; Misumi, Y.; Suzuki, A.; Hoshida, H.; Akada, R., Triple gene expressions in yeast, Escherichia coli, and mammalian cells by transferring DNA fragments amplified from a mother yeast expression plasmid. J Biosci Bioeng. doi: 10.1016/j.jbiosc.2022.03.002, 2022

Kikuta, H.; Goto, S., Kondo, M.; Akada, R,; Hoshida, H., Identification of essential intron sequences that enhance gene expression independently of splicing in the yeast Saccharomyces cerevisiae, BBA-Gene Regulatory Mechanisms, 1865(1):194784. doi: 10.1016/j.bbagrm.2021.194784, 2021.

Kondo, W,; Kitagawa, T,; Hoshida, H,; Akada, R.; Miyakawa, Morphological Changes of Mitochondria and Actin Cytoskeleton in the Yeast Saccharomyces cerevisiae During Diauxic Growth and Glucose Depletion Culture.,Cytologia, 87:157-162. doi: 10.1508/cytologia.87.157, 2022

Abdel-Banat, B. M. A., Hoshida, H., Akara, R., Various short autonomously replicating sequences from the yeast Kluyveromyces marxianus seemingly without canonical consensus., Curr. Res. Microbial Sci. 2, 2021, https://doi.org/10.1016/j.crmicr.2021.100053.

Sato, T.; Hoshida, H.; Akada, R., Inhibition of distinct proline- or N-acetylglucosamine-induced hyphal formation pathways by proline analogs in Candida albicans., BioMed Research International, 2020: 7245782, 2020.

Hoshida, H.; Kagawa, S.; Ogami, K.; Akada, R., Anoxia-induced mitophagy in the yeast Kluyveromyces marxianus.,FEMS Yeast Res., 20(7):foaa057, 2020.

Fujiwara, N.; Shibutani, S.; Sakai, Y.; Watanabe, T.; Kitabayashi, I,; Oshima, H.; Oshima, M.; Hoshida, H.; Akada, R.; Usui, T.; Ohama, T.; Sato, K., Autophagy regulates levels of tumor suppressor enzyme protein phosphatase 6., Cancer Sci., 111:4371-4390, doi: 10.1111/cas.14662, 2020.

Misumi, Y.; Nishioka, S.; Fukuda, A.; Uemura, T.; Nakamura, M.; Hoshida, H.; Akada, R., YHp as a highly stable, hyper-copy, hyper-expression plasmid constructed using a full 2-μm circle sequence in cir0 strains of Saccharomyces cerevisiae., Yeast, 36:249-257, 2019.

Fujisawa, K.; Takami, T.; Nagatomo, T.; Fukui, Y.; Hoshida, H.; Saeki, I.; Matsumoto, T.; Hidaka, I.; Yamamoto, N.; Sakaida, I., Usefulness of adult medaka fish as a model for the evaluation of alcoholic fatty liver., Alcohol, 77:147-154, 2019.

Hoshida, H.; Kidera, K.; Takishita, R.; Fujioka, N.; Fukagawa, T.; Akada, R., Enhanced production of extracellular inulinase by the yeast Kluyveromyces marxianus in xylose catabolic state., J. Biosci. Bioeng., 125:676-681, 2018.

Nakamura, M.; Aihara, J.; Hoshida, H.; Akada, R., Identification and Mutational Analysis of Escherichia coli Sorbitol-Enhanced Glucose-Repressed srlA Promoter Expressed in LB Medium by Using Homologous Recombination and One-Round PCR Products., Mol. Biotechnol., 60:912-923, 2018.

Ortiz-Merino, R. A.; Varela, J. A.; Coughlan, A.Y.; Hoshida, H.; da Silveira, W. B.; Wilde, C.; Kuijpers, N. G. A.; Geertman, J. M.; Wolfe, K. H.; Morrissey, J. P., Ploidy Variation in Kluyveromyces marxianus Separates Dairy and Non-dairy Isolates., Front. Genet., 9:94, 2018.

Varela, J. A.; Montini, N.; Scully, D.; Van der Ploeg, R.; Oreb, M.; Boles, E.; Hirota, J.; Akada, R.; Hoshida, H.; Morrissey, J. P., Polymorphisms in the LAC12 gene explain lactose utilization variability in Kluyveromyces marxianus strains., FEMS Yeast Res., 17, fox021, 2017.

Hoshida, H.; Kondo, M.; Kobayashi, T.; Yarimizu, T.; Akada, R., 5´-UTR introns enhance protein expression in the yeast Saccharomyces cerevisiae., Appl. Mcrobiol. Biotechnol., 101:241-251, 2017.

Nakamura, M.; Suzuki, A.; Akada, J.; Tomiyoshi, K.; Hoshida, H.; Akada, R., End Joining-Mediated Gene Expression in Mammalian Cells Using PCR-Amplified DNA Constructs that Contain Terminator in Front of Promoter., Mol. Biotechnol., 57:1018-1029, 2015.

Suzuki, A.; Fujii, H.; Hoshida, H.; Akada, R., Gene expression analysis using strains constructed by NHEJ-mediated one-step promoter cloning in the yeast Kluyveromyces marxianus., FEMS Yeast Res., 15, fov059, 2015.

Nakamura, M.; Suzuki, A.; Akada, J.; Yarimizu, T.; Iwakiri, R.; Hoshida, H.; Akad,a R., A Novel Terminator Primer and Enhancer Reagents for Direct Expression of PCR-Amplified Genes in Mammalian Cells., Mol. Biotechnol., 57:767-780, 2015.